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KMID : 1134819970260050901
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Journal of the Korean Society of Food Science and Nutrition
1997 Volume.26 No. 5 p.901 ~ p.907
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Effects of Green Tea Catechin on Cytochrome P450, Xanthine Oxidase Activities in Liver and Liver Damage in Streptozotocin induced Diabetic Rats
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Park Gyu-Young
Rhee Soon-Jae
Im Jung-Gyo
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Abstract
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The purpose of this study was to investigate the effects of green tea catechin on free radical generation system and peroxidative damage in the liver of streptozotocin(STZ)-induced diabetic rats. Sprague-Dawley male rats weighing 150±10 gm were randomly assigned to one normal and three STZ-induced diabetic groups. Diabetic groups were classified to catechin free diet(DM-0C group), 0.5% catechin diet(DM-0.5C group) and 1% catechin diet(DM-1C group) according to the levels of dietary catechin supplementation. Diabetes was experimentally induced by intravenous injection of 55㎎/㎏ of body wt of STZ in citrate buffer(pH 4.3) after feeding of three experimental diet for 4 weeks. Animals were sacrificed at the 6th day of diabetic states. Activities of serum glutamic oxaloacetic transaminase(GOT) and the glutamic pyruvic transaminase(GPT) in DM-0C groups were higher than those of the normal group, and those in catechin supplementation group were similar to those of the normal group. Liver lipid peroxide values increased by 153%, 49% and 27% in DM-0C, DM-0.5C and DM-1C groups, repectively, compared with the normal group. Liver XOD activity was increased in DM-0C, but was not significantly different in catechin supplementation groups compared with the normal group, and liver cytochrome P_(450) contents was similiar to result of XOD activity. In electron microscopic examination of liver, lysosome was relatively scatterd in DM-0C and DM-0.5C group and preserved normal shapes in DM-1C group. The present results indicate that STZ-induced diabetic rats are more sensitive to oxidative stress, leading to the acceleration of lipid peroxidation process, but this was reduced by anti-oxidative effect of high level of dietary catechin. It is concluded that dietary catechin serves as powerful antioxidant against lipid peroxidation in diabetic rats.
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KEYWORD
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diabetes mellitus, cytochrome $P_{450}$, xanthine oxidse, peroxidative damage, catechin
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